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Primary cells were cultured in X-Vivo 15 Serum-free Hematopoietic cell medium (Lonza, Basel, Switzerland, Cat#: 02-060Q) supplemented with granulocyte-macrophage colony-stimulating factor (GM-CSF) (Cell Signaling Technology, Danvers, MA Cat#: 8930) at 10 ng/mL, interleukin 3 (IL3) (Cell Signaling Technology, Cat#: 8918) at 50 ng/mL, recombinant human thrombopoietin (TPO, Biolegend, San Diego, CA, Cat#: 712602) at 50 ng/mL, stem cell factor (SCF) (Cell Signaling Technology, Cat#: 9907) at 25 ng/mL, FLT3 ligand (FLT3L) (Peprotech, Rocky Hill, NJ, Cat#: 300-19) at 50 ng/mL, interleukin 6 (IL6) (Cell Signaling Technology, Cat#: 9954) at 10 ng/mL, and granulocyte colony-stimulating factor (G-CSF) (Cell Signaling Technology, Cat#: #8930) at 1 ng/mL